Annotation Studio is an open source, web-based annotation application that integrates a powerful set of textual interpretation tools behind an intuitive and easy-to-use interface. Users can upload their own texts, and annotate with styled text, video, images, and weblinks. To date, the project has been used with great success in disciplines such as Writing, Literature, Foreign Languages, Anthropology, Film and Media Studies, and others at institutions including Harvard, Yale, Stanford, MIT, Barnard College, and Washington University.